掲載内容
◆Octet System 概要
◆スクリーニングアッセイ ワークフロー
◆スクリーニングアッセイ実例
◆詳細はカタログをダウンロードしご覧いただくか、お気軽にお問い合わせ下さい。
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| ドキュメント名 | 抗体医薬品・診断薬開発に活用するOctetシステムによる効率的なスクリーニングアッセイ |
|---|---|
| ドキュメント種別 | 製品カタログ |
| ファイルサイズ | 4.1Mb |
| 登録カテゴリ | |
| 取り扱い企業 | ザルトリウス・ジャパン株式会社 (この企業の取り扱いカタログ一覧) |
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このカタログの内容
Page1
Octet
2020/11/17
Page2
Agenda
Octet System
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Agenda
Octet System
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Association
AB A
A Y
Y B
B
YY Y Y Y Dissociation
A: B: AB:
Time
Kon Kon: Kdis
A + B AB
Kdis Kdis: KD = Kon KD:
▪
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Binding (nm)
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ELISA
▪
▪
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▪
▪
▪
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Real-Time Kinetics Improvements Over ELISA Techniques
▪ KD Association Dissociation KD = Affinty
Example A 1.00E+04 1.00E-02 1.00E-06
Example B 1.00E+02 1.00E-04 1.00E-06
▪ Octet
KD
Kon Kdis
ELISA
▪ ELISA
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Over 1000 screening samples!
high-throughput
screening by BLI
Octet HTX
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The ForteBio Octet Systems
Octet RED Series
Analyte MW: > 150 D
KD range: 1 mM - 10 pM
K2 RED96e RED384
Octet QK Series
Analyte MW: > 5 kD HTX
KD range: 10 mM - 1 nM
QKe
• 2-channel • 8-channel • 16-channel • 96-channel read head
• 96-well plates • 96-well plates • 96 & 384-well plates • 96 & 384-well plates
• >180µL assay vol. • >180µL assay vol. • >40µL assay volume • >40µL assay volume
• Automation Compatible • Automation Compatible
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◆ m m
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Dip and Read
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Bio-Layer Interferometry (BLI)
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（BLI: Bio-Layer Interferometry）
100%
0
Distance Wavelength (nm)
between the
two reflecting
surfaces = ℓ
Intensity λ =ƒ(λ, ℓ)
Function of density and optical thickness
Time
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nm shift Relative Intensity
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Dip and Read
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Breakthrough Technology: User-Selectable Read Head
8 or 16 sensors – RED96/384 sensitivity
32 or 48 sensors – QK384 sensitivity
96 sensor – QK384 sensitivity
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Model Channel Plate Sample volume Robot
8, 16
HTX 32, 48, 96 96 or 384 well 40 mL / well
RED384 8, 16 96 or 384 well 40 mL / well
RED96 8（ 96 well 200 mL / well
＜Robot ＞
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Octet
▪
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▪
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▪
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Octet ELISA
▪
pH
▪
▪
KD Kon, Koff
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Agenda
Octet System
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Octet
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Applications in Lead Screening Workflow
1o • Binding to target protein (yes/no)
>1,000s mAbs
SCREEN
• Off-rate Ranking
2o
• Epitope Binning
500-1000 mAbs SCREEN
• Functional or Cell-based Assays
3o • Affinity Ranking
SCREEN • Concentration
~100-200 mAbs
• Concentration (small scale-up)
HIT TO LEAD • Ligand Binding Activity
SELECTION
~50 mAbs
• FcRN/FCg Binding
LEAD • Kinetic Characterization
OPTIMIZATI
~5-10 mAb Lead ON • Epitope Mapping
Final leads <10
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Octet
Octet RED96e Octet RED384 Octet HTX
# Channels per Read 8 16 96
Molecular Weight Range > 150 Da > 150 Da > 150 Da
Microplate Positions 1 2 2
Robot Compatible No Yes Yes
Sample Vessel Formats 96 96 / 384 96 / 384
Minimum Sample
180 µL per well 40 µL per well 40 µL per well
Volume
Affinity range
1 mM to 10 pM 1 mM to 10 pM 1 mM to 10 pM
(approximate)
Throughput (96 samples
30 min 15 mim 2 min
quantitation)
Throughput (96 samples 3 h 40 min (need
1 h 50 min 18 min
kinetics koff screening) plates replace)
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