Infographics「Detecting SARS-CoV-2 Antibodies in Serum and Plasma Samples」

DETECTING SARS-COV-2 ANTIBODIES IN SERUM AND PLASMA SAMPLES Convalescent plasma therapy is a potential treatment under investigation where antibodies from recovered patients are transfused to current COVID-19 patients with the intent to help them fight the infection and buy time until their immune system can produce antibodies. However, not all recovered persons have the same amount of antibody titers suitable for such transfusions. Reliable, simple, and rapid methods are needed to assess relative antibody titers in plasma samples. HOW WILL YOU KNOW IF PLASMA ANTIBODY LEVELS Plasma Low High ARE SUFFICIENT? separation Coronavirus Detect from blood antibodies antibody levels HOW TO DETECT PLASMA ANTIBODIES USING THE OCTET SYSTEM Step 1: Prepare a Step 2: Detect antibody binding signals Step 3: Analyze binding data coronavirus antibody specific biosensor Biosensor Octet Patient 3 Positive control RED96e Biosensor Patient 2 Instrument Patient 4 Viral Patient 1 Negative control protein Time (sec) Immobilize a virus protein Dip the coronavirus biosensor into Quantitate relative antibody such as the receptor binding diluted patient plasma or serum samples. levels in plasma or serum. domain (RBD) of the SARS Block the biosensor with non-relevant CoV-2 spike protein. serum or blocking buffer if needed to prevent non-specific binding. WHAT THE DATA TELLS YOU A RANGE OF BLI PLATFORMS TO MEET YOUR NEEDS Antibody levels P5 Med Octet HTX Instrument P4 • 96-channel fully automated Hi • Samples in 96/384-well plates P3 Med • Robot compatible P2 Neg Octet RED384 Instrument P1 Lo • 16-channel fully automated Positive control • Samples in 96/384-well plates 0 20 40 60 80 100 120 • Robot compatible % Binding Octet RED96e Instrument HOW DO OCTET SYSTEMS • 8-channel fully automated HELP YOU GET DATA FAST? • Samples in 96-well plates Octet K2 Instrument • Rapid test generating real-time data • 2-channel fully automated • Simple workflow and easy to use • Samples in 96-well plates • Low cost assay development BLItz Instrument • Wide-range of available biosensor • Single channel manual system chemistries to immobilize virus • Sample consumption from proteins 4–250 µL Learn more about how Octet systems are used in COVID-19 research at www.fortebio.com/covid19research Binding (nm)