DASbox Mini Bioreactor System for cell culture and microbiology

Most Compact DASbox® Mini Bioreactor System for cell culture and microbiology

Epitome of compact: A 24-fold DASbox system takes up only 1.8 m (6 ft) of bench space. Parallel Process Development Parallel operation to shorten your time-to-market Compact design and small working volumes save lab The smart design of the Eppendorf DASbox makes the 4-fold space and valuable resources units ideal for parallel processing of up to 24 bioreactors. Lab space is critical: The DASbox requires only 7.5 cm (3 in) Together with the DASware® software solutions, the DASbox of bench space per bioreactor maximizing use of lab space supports process development following the Quality by while being expandable in 4-fold unit increments to increase Design (QbD) approach by providing comprehensive capacity as needed. Small working volumes of 60 – 250 mL information management, integration of third-party make it a perfect fit for clone/cell line screening, media analyzers, and Design of Experiments (DoE) tools as well as optimization and small scale process development. remote access. One solution for all: fermentation and cell culture Suitable for microbiology and cell culture, the DASbox features advanced process control and precise monitoring of all critical process parameters. DASbox® single-use solutions: the BioBLU® 0.3 The Eppendorf BioBLU 0.3 Single-Use Bioreactors for use with the DASbox offer a proven rigid-walled single- use bioreactor portfolio—with vessels for cell culture and microbial applications, including high cell density fermentation and cultivation of stem cells. As the smallest member of the BioBLU Single-Use Bioreactor family the BioBLU 0.3 allows for scalability from 65 mL up to 40 L working volume. Autoclavable BioBLU 0.3 glass bioreactors Single-Use Bioreactor Fermentation   Suspension cells   Stem cells   BioBLU 0.3c BioBLU 0.3f BioBLU 0.3sc for cell culture for microbiology for cell aggregates Watch our video: www.eppendorf.com/dasbox-video

Small, but powerful. LC Color Display All vessels and parameters at a glance. Integrated Feeding and Monitoring Variable speed pumps and standard pH and DO sensors (redox or level options and optical pH sensors available) Advanced Temperature Control Liquid-free temperature control unit for easy handling TMFC Gassing Integrated mass flow- controlled gas mixing system for continuous mixing of air, N2, O2 and CO2

Technical data DASbox® Mini Bioreactor System for Cell Culture DASbox® Mini Bioreactor System for Microbiology Parallel bioreactors up to 24 up to 24 Software DASware control, other DASware optional DASware control, other DASware optional Vessels Glass and single-use vessels Glass and single-use vessels Working volumes 60 – 250 mL (glass)/100 mL – 250 mL (single-use) 60 – 250 mL (glass)/65 – 250 mL (single-use) Drive Overhead drive Overhead drive Impellers Marine (glass)/pitched blade (single-use)/8-blade Rushton-type (glass, single-use) Agitation speed ranges 20 – 2,500 rpm (glass)/ 20 – 2,500 rpm (glass)/20 – 2,000 rpm (single-use) 20 – 500 rpm (single-use, pitched blade impeller)/ 20 – 200 rpm (single-use, 8-blade impeller) Temperature control Liquid-free heating and cooling (Peltier) Liquid-free heating and cooling (Peltier) Standard temperature range 10 – 60°C at 25°C RT 10 – 60°C at 25°C RT Feeding lines per vessel 2 (standard)/4 (optional) 2 (standard)/4 (optional) Standard feed rates (depending 0.3 – 9.5 mL/h to 13 – 420 mL/h 0.3 – 9.5 mL/h to 13 – 420 mL/h on tube diameter) Gas flow control TMFC; overlay and/or sparger TMFC Standard gas mixing Air, N2, O2 and/or CO2 Air, N2, O2 and/or CO2 Standard gas flow rates 0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO2 0.2 – 25 sL/h, 0.2 – 18 sL/h CO2 pH control CO2/base, and other set-ups Acid and/or base, and other set-ups DO control Cascade (O2 concentration, gas flow rate) Cascade (agitation speed, O2 concentration, gas flow and other set-ups rate), and other set-ups ORP (redox) measurement - Optional (select redox or level) Level/foam Optional Optional (select redox or level) OD measurement Optional (DASGIP OD4) Optional (DASGIP OD4) Exhaust condensation Liquid-free (Peltier) Liquid-free (Peltier) Exhaust analysis - Optional (DASGIP GA4) Specifications are subject to change without notice Ordering information Description Order no. (system Order no. (system for with glass vessels) single-use vessels) DASbox® Mini Bioreactor System for Cell Culture Applications, max. 5 sL/h gassing 4-fold system 76DX04CC 76DX04CCSU 8 fold system 76DX08CC 76DX08CCSU 16-fold system 76DX16CC 76DX16CCSU 24-fold system 76DX24CC 76DX24CCSU DASbox® Mini Bioreactor System for Microbial Applications, max. 25 sL/h gassing 4-fold system 76DX04MB 76DX04MBSU 8-fold system 76DX08MB 76DX08MBSU 16-fold system 76DX16MB 76DX16MBSU 24-fold system 76DX24MB 76DX24MBSU BioBLU® 0.3c Single-Use Bioreactor, cell culture, 1 pitched-blade impeller, sterile, 4-pack 1386100000 BioBLU® 0.3c Single-Use Bioreactor, cell culture, 1 pitched-blade impeller, optical pH, sterile, 4-pack 1386100200 BioBLU® 0.3f Single-Use Bioreactor, fermentation, 2 Rushton-type impellers, sterile, 4-pack 1386100100 BioBLU® 0.3sc Single-Use Bioreactor, cell culture, 8 blade impeller, optical pH, sterile, 4-pack 1386100600 Your local distributor: www.eppendorf.com/contact Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany eppendorf@eppendorf.com · www.eppendorf.com www.eppendorf.com Eppendorf®, BioBLU®, and the Eppendorf Brand Design are registered trademarks of Eppendorf SE, Germany. DASGIP®, DASbox® and DASware® are registered trademarks of DASGIP Information and Process Technology GmbH, Germany. All rights reserved, including graphics and images. Copyright © 2022 by Eppendorf SE. Order no. A761 311 020/GB4/0.5T/0422/EBC/STEFF · Carbon neutrally printed in Germany