BioFlo 120 bioprocess control station

Keep Growing BioFlo® 120 bioprocess control station

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BioFlo® 120 3 »Performance meets value.« The Eppendorf BioFlo 120 offers simplicity and ease of use, without sacrificing capability. No matter if you are in an academic, governmental or industrial research setting, or working with bacteria, yeast, fungi, mammalian, insect or plant cells, the BioFlo 120 is an attractive solution to meet your needs–all at an affordable price. Feature-packed and future-proof > Scale-up from 250 mL to 40 L on a wide variety of > Universal connections for digital Mettler Toledo® ISM® autoclavable and Eppendorf BioBLU® Single-Use sensors or analog sensors offer unsurpassed flexibility Bioreactors > User-defined DO cascades offer process flexibility > New Auto Culture modes offer process control for > Automatic gas mixing algorithms for simplified control microbial and cell culture applications at the touch of a > View your entire process with expanded trend screen button > Access your data from anywhere with Eppendorf SCADA > Ready for process. Unbox and install in minutes platforms, IP network, and remote monitoring capabilities > Save critical lab space with a minimal footprint

4 BioFlo® 120 Connection for interchangeable direct- and magnetic-drive motors; Easy-to-read 7” integrated magnetic drive capable of clockwise and touchscreen monitor counterclockwise rotation for simplified impeller selection Three user-defined analog input /output All vessel connections. Select connections between 0-5V, 0-10V, located on the and 4-20 mA depending side–set up and on the device ready to go in minutes High-precision thermal mass flow controller (TMFC) or rotameter for gas flow control. Standard automatic IP21 rated enclosure mixing of four for protection of independent sparge gas sensitive electronics supplies and cleanability Water recirculation module Three front-mounted fixed-speed pumps provides precise temperature with industry-standard easy load pump control and exhaust heads for convenient liquid addition/ condensing removal

BioFlo® 120 5 BioBLU® single-use advantage Sophisticated software > The rigid-walled design advantage: > Eppendorf Bioprocess Control Software offers real time, > Sets up in minutes, no inflation necessary local process control with integrated touchscreen > No more tears, pits, or folds as seen in bag installations > Optional Eppendorf SCADA platforms (BioCommand®, > Consistent leachables and extractables profile with DASware®) provide high level process control capabilities, single-layer molded polymer vs. multi-layer bag design and secure database management > Scale from 250 mL to 40 L with BioBLU Single-Use > IP networking provides remote access through PC or Bioreactors mobile device > BioBLU f Single-Use Bioreactors designed for high density fermentation > Eppendorf exclusive BioBLU 5p with Fibra-Cel® Disks for continuous and perfusion processes Scalable solutions Unlimited applications > Perfectly suited for research and development > Grow any cell type you can think of: Microbial, insect, > Scale-up and scale-down modeling plant, fungal, mammalian, and stem cells > Growth of seed to pilot-scale cultures in a working volume > Unlimited process flexibility: Batch, fed-batch, continuous, range of 250 mL to 40 L or perfusion; supports high-density, micro-aerobic, and > Blends into the Eppendorf bioreactor and fermentor anaerobic fermentation, secreted products production, and portfolio covering working volumes of 65 mL to 2,400 L, process development for cell and gene therapies for comprehensive scalability Find more detailed information, including video presentations, an online configurator and a 360° product show on our website. Visit www.eppendorf.group/BioFlo-120 or easily scan the QR code beside.

Liquid Handling Cell Handling Sample Handling To make your job in the lab easier and more efficient – with this goal in mind we are developing products and solutions in the areas of Liquid Handling, Cell Handling, and Sample Handling. Visit the Eppendorf Handling Solutions online sphere and dive into the area of your choice, learn new things, and have fun as well: www.eppendorf.com/handling-solutions Liquid Handling Cell Handling Sample Handling In 1961, Eppendorf launched For handling cells, in addition Sample Handling encompasses the first piston-stroke pipette. to manipulators and injectors, many different work processes Today, our broad product incubators and consumables for and steps: centrifugation, offerings in Liquid Handling cultivation as well as complete heating, freezing, mixing, range from manual pipettes to bioreactor systems for cell amplification, and analysis of electronic pipettes, dispensers culture applications are available. samples. Eppendorf offers the and burettes to automated Corresponding detection systems devices and consumables needed pipetting systems. are offered for subsequent analysis. for each of these steps. Eppendorf Easypet® 3 New Brunswick S41i Centrifuge 5920 R Experience a new dimension of electronic The only CO2 incubator with an Eppendorf Experience extraordinary high capacity pipetting with complete speed control and shaker inside. in a very compact and ergonomic product the utmost precision. design. > Precise control of temperature, shaking > Intuitive and convenient speed adjustment speed and CO2 for stable culture conditions > S wing-bucket rotors and adapters simply done with the tips of your fingers > E asy-to-clean chamber design and accomodate tubes and bottles from 0.2 mL > Lightweight, well-balanced and ergonomic 120°C disinfection cycle saves time and to 1,000 mL design that allows for fatigue-free pipetting effectively eliminates contamination > F ixed-angle rotors and plate rotor options epMotion® 5075m Concerned about Bacteria in The most flexible member of our family of automated liquid handling systems. Cell Culture? > Mixing, temperature control and magnetic Find useful tips on bead separation abilities > O ptical sensor verifies labware, tips and www.eppendorf.com/cellexperts volumes before the run

BioFlo® 120 7 Bioreactor Specifications Autoclavable bioreactors Bioreactor 1 L 2 L 5 L 10 L Total volume 1.3 L 3.0 L 7.5 L 14.0 L Working volume 0.4 – 1.0 L 0.8 – 2.2 L 2.0 – 5.6 L 4.0 – 10.5 L Vessel type Water-jacketed or heat-blanketed Material Borosilicate glass, 316L stainless steel Impellers Direct drive or magnetic drive Rushton-type, pitched blade, marine or spin filter Autoclave dimensions Heat-blanketed Outer diameter (OD) 20.3 cm 20.3 cm 29.8 cm 29.8 cm 8.0 in 8.0 in 11.7 in 11.7 in Height (without exhaust filter) 54.0 cm 54.0 cm 61.0 cm 68.0 cm 21.3 in 21.3 in 24.0 in 26.9 in Water-jacketed Outer diameter (OD) 24.1 cm 24.1 cm 29.8 cm 29.8 cm 9.5 in 9.5 in 11.7 in 11.7 in Height (without exhaust filter) 48.9 cm 56.5 cm 64.8 cm 76.8 cm 19.3 in 22.3 in 25.5 in 30.3 in Number of head plate ports 6 mm 1 6 7 7 12 mm 9 7 8 8 19 mm 0 0 1 1 Total 10 13 16 16 Recommended sensor lengths (mm) Sensor pH (analog)1 200 225 325 425 pH/redox (digital)1 225 325 425 DO (analog)1 160 220 320 420 DO (digital/optical)1 220 320 420 Redox (analog)1 200 325 325 425 CO2 (digital)1 320 320 320 Single-use bioreactors Bioreactor BioBLU 1c/f BioBLU 3c/f BioBLU 5c BioBLU 5p BioBLU 14c BioBLU 50c Total volume 1.8 L 5 L 5 L 5 L 14 L 50 L Working volume 0.25 – 1.25 L2 1.25 – 3.75 L 1.25 – 3.75 L 3.75 L 3.5 – 10.5 L 18 – 40 L Vessel type Rigid-walled, stirred-tank Impellers Magnetic drive BioBLU c: pitched blade/BioBLU p: packed-bed/BioBLU f: Rushton-type Recommended sensor lengths (mm) pH (EC)3 220 225 225 120 425 625 DO3 220 225 225 120 355 526 Redox3 220 225 225 120 425 625 CO 3 2 220 220 220 120 320 N/A 1 Installation may require compression fitting for optimal fit and depth (M1273-5040), 2 x included with Vessel Connection Kit. 2 BioBLU 1c: 425 mL minimal working volume when used with vessel stand and heat blanket 3 Installation may require compression fitting for optimal fit and depth (1386010200). Specifications subject to change.

BioFlo 120 Specifications Control Station Dimensions (W x D x H) 24.7 x 55.9 x 62.9 cm (9.7 x 22 x 24.8 in) Net weight 14.8 kg (32.7 lb) Touchscreen 7 in projected capacitive touchscreen Communication 2 x USB (software updates, serial communication) Ethernet (SCADA, IP Network) Utility Connection Requirement Electrical IEC-C14 (with regional plug types) 100 – 120/208 – 240 (± 10 %) V, 50/60 Hz, 10 A, Single Phase Water Quick-connect 10 psig (0.69 barg) Gas supply (air, O2, N2, CO2) Push-connect fittings accept 1/4 in Autoclavable Single-use tubing or hose barb fitting 10 psig (0.69 barg) 6 psig (0.44 barg) Exhaust 0.5 psig (0.035 barg) Operating conditions 10 – 30°C, up to 80 % RH, non-condensing Agitation Direct drive 1 L: 25 – 1,500 rpm 3 L, 5 L, 10 L: 25 – 1,200 rpm Magnetic drive (autoclavable vessels) 1 L, 3 L, or 5 L: 5 – 500 rpm 10 L: 5 – 150 rpm Magnetic drive (single-use vessels) BioBLU 1f & 3f: 5 – 1,200 rpm BioBLU 1c: 5 – 500 rpm BioBLU 3c, 5c, 5p & 14c: 5 – 200 rpm BioBLU 50c: 5 – 150 rpm Temperature Autoclavable 1, 2, 5 L: 8°C above coolant to 45°C above ambient (0°C – 70°C absolute)* 10 L: 8°C above coolant to 40°C above ambient (0°C – 65°C absolute)* BioBLU Single-Use Vessels BioBLU c vessels: 5°C above ambient to 40°C* BioBLU f vessels: 5°C above coolant to 45°C* Sensor type Pt100 Gas supply Sparge 1 TMFC (0.002 – 1 SLPM or 0.01 – 5 SLPM or 0.04 – 20 SLPM) or 1 rotameter (multiple options available); ring or microsparger Sensors Communication Control range pH Analog or digital Mettler Toledo ISM 2 – 12 DO Analog or digital Mettler Toledo ISM 0 – 200 % (air saturation) Optical DO Digital Mettler Toledo ISM 0 – 200 % (air saturation) Redox Analog or digital Mettler Toledo ISM (-)2000 mV – (+)2000 mV CO2 Digital Mettler Toledo ISM 0 – 100 % Pumps Pump head Fixed speed Pumps 1, 2, & 3 Watson-Marlow 114DV 30 rpm (0 – 100 % duty cycle) * Requires 115/230 V line voltage. Specifications cannot be guaranteed with operation at alternative line voltages. Specifications subject to change. Your local distributor: www.eppendorf.com/contact Eppendorf SE · Barkhausenweg 1 · 22339 Hamburg · Germany eppendorf@eppendorf.com · www.eppendorf.com www.eppendorf.com Mettler Toledo® and ISM® are registered trademarks of Mettler Toledo AG, Switzerland. Watson-Marlow® is a registered trademark of Watson-Marlow Limited, UK. Eppendorf®, the Eppendorf Brand Design, BioBLU®, Easypet®, and epMotion® are registered trademarks of Eppendorf SE, Germany. DASware® is a registered trademark of DASGIP Information and Process Technology GmbH, Germany. BioFlo® and BioCommand® are registered trademarks of Eppendorf, Inc., USA. All rights reserved, including graphics and images. Copyright © 2022 by Eppendorf SE. Order No.: AN01 311 020/GB7/0.5T/0422/EBC/STEFF · Carbon neutrally printed in Germany