Test Paper General Bacteria Coli form Group

Test Paper General Bacteria Coli form Group Easy Fast Long Seller Why choose SIBATA? Easy SIBATA’s test paper is easy to This product is very simple testers treated with certain use without technical knowl- microbe growth promoters for checking bacteria edge. contamination condition. You can test not always This is a simple easy test kit liquid sample but also solid surface sample. for daily use. Anybody can simply use this paper in the field without Disposal work is easyer than special equipment or skills. dish Usage Example of General Bacteria Fast *General food except daily products, cooking ware, and so on. This can be measured rela- tively faster. Usage Example of Coli form Group Incubation time is 24hours *Testing milk, drinking water, fresh food, frozen food for general bacteria and 15 and other foods drinks. hours for coli form group. *Testing table ware and cooking ware or other facilities for food processing. Long Seller *Testing swimming pool water, public baths, and other. Since starting selling theprod- *Testing industrial effluent, stockyard effluent, and ucts in 1980, it is used as a other. screening kit as an alternative to the field of hygiene man- agement.

measuring method Liquid Semifluid Solid Solid Surface Sample Sample Sample Sample ①Put *sterile physi- Shake up the sample Homoginize the sample ological saline into a Shake up and Physiological and physiological sterilized container, saline saline by stomacher or then soak a sterilized homogenizer. cotton bud or gauze in it to make it moisten. ②Wipe out the surface of the sample with the bud or gauze. ③Shake up the bud or Gauze in the container and take it out. The sterile physi- ological saline in the container becomes a sample. ① ② ●Use Pipets ●Dip it into the sample water cut cut sample water Open the polyethylene pouch, pinch the top of Drop 1mL of the sample water on Dip it into the sample water, pull it out and shake off the extra water. test paper ant take it the test paper. Put the sample test paper into the polyethylene pouch, cut the perforation line and out. throw away the top. ③ ④ Incubater ⑤ （37℃） Test Paper lights Push the air out of the pouch. Then seal the Put It In a thermostat and incubate it at fastener. 35-37℃. Count the number of spots(colonies). Coli form Group・・・15 hours General Bacteria・・・24 hours *Sterile distilled water or cold water after boiling can be used as sterile physiological saline.

Frequently asked questions Q:What are ingredients impregnated in the paper? General Bacteria : cellulose fiber, peptone, meat extract, glucose, yeast extract, TTC, Coli form group : cellulose fiber, peptone, Q:How long is the expiration date? lactose, pH control chemicals, yeast extract, Approximately 2 years (if in a cool and dark sodium deoxycholate, TTC, place). It is described on the package. Q：Is incubating time for general bacteria is 24 hours at 35 ℃ and 37℃? There is temperature gap of 2℃ but both incubation times are 24 hours.Warm and genial temperature like body temperature is appropri- Q:How can I dispose a used test paper? ate. Please let the paper dry and dispose as instructed in your local government, and besides, it is much easier than Petri dish. Q：Why does a paper become blurry red? Because of too many bacteria and overlap Q:Can anyone use this paper? considerably, whole surface becomes red coloration. Yes, this is very simple kit so for those who does not have a technical knowledge. Special equipment and skills are not required and Q：What should be prepared for testing? incubation time is short. You can check daily hygiene management without any circum- Please use your thermometer and colony stance. counter (if necessary). If you don’t have them, we highly recommend you our CALBOX CB-101 and handy colony counter. Code No. / Model 080510-32 / CB-101 Thermostat for test of Bacteria called Heater 20W “CALBOX” model CB-101 is a small Setting Temp. 37℃(fixed) thermo-stated incubator used for general Temp. display Thermometer bacteria culture and coli form group. No of papers 40 sheets acceptable Dimensions 240W x 160D x 82Hmm Code No. 051250-03 This is a stylus pen called Indication digital 5 fitures “handy colony counter” to Battery lithium battery(CR-2032) 3V count colonies on a test Dimensions 181W x 28D x 16Hmm paper. Weight approx 35g Accessories 2 permanent markers(1 black, 1 blue), 1battery

Estimation of General Bacteria The main ingredient of this test paper is TTC used as color a former. Therefore, the test can not indicate all the various kinds of general bacteria, and by the following way, the number of general bacteria is given as a standard value. For example, in Japan, the samples are classified into 6 kinds, the general bacteria number of every kind is valued by the product of spot number by a coefficient. When this general bacteria test paper is used in other regions or countries, water samples must be simultaneously examined with this test paper and standard method(See: classified table of sample --- Spot number limited to 10 – 100). Then, from the repeated results, each coefficient will be decided for an easy test method.If spot number is less than ten, extend the incubation time for another 24 hours (total 48 hours), and the general bacteria number will be nearly as it appears. Sample Classified Table Example: in Japan No. Kinds of Water Coefficient 1 Water from spring or well, industrial water, and other. 4 2 Water from river, fountain, and other. 2 3 Water from bath, and other. 3 4 Rotten water, water from pools or ponds, and other. 3 5 Bean curd or other foods soaked water in apackage, and other. Especially in summer 10 6 Water mixing oil (cutting oil, and other) 10 Dilute sample to 1,000 times of original before use. If spot number is more than 100, dilute the original by pure water and experiment on it once again. The diluting water must be sterilized(boiled). The spot number is 48. (between 10 and 100) Sample --- Bean curd soaked water in a package The general bacteria number = 48×10 = 480 Dillution times Spot number on test paper Spot number on plate culture (Classified Table of Samples) 0(original) thin red, numerous numerous 1,000 times thin red, numerous numerous 10,000 times red, small spot 48 small spot=500 Estimation of Coli form Group After incubation, the coli form group appears as red spots. Generally, 5 sheets of test paper per sample should be used, the estimation is performed by mean value of spot number. When the test papers are dried at 60℃ to 70℃ after the test, they can be conserved. Specification Item Test Paper for General Bacteria Test Paper for Coli Form Group code 080510-302 080510-301 component 100 sheets 100 sheets culture time 24 hours 15 hours Specifications, and appearance described in this document are based on information as of March 25, 2015. They are subject to change without notice for improvement of the product. 1-1-62, Nakane Soka-City, Saitama, Japan TEL:+81-48-933-1582　FAX:+81-48-933-1591 E-mail:overseas@sibata.co.jp http://www.sibata.co.jp/english/ 201503K