Osmium Coater

Discover New Features Comparison Between Sputter Coater of Your Samples and Neoc Artemia salina larvae were freeze-dried and coated by a sputter coater and the Neoc. The images below show the difference. The one coated by the Neoc clearly reveals more details. Sputter Coater Neoc (Platinum Palladium) (Osmium Tetroxide) (Coating Time: 20 sec) (Coating Time: 20 sec) Accelerating Voltage 10kV Accelerating Voltage 10kV Sand Star 5kV x 10,000 The image is provided by JEOL Ltd. Development Concept Meiwafosis Co., Ltd. has been seeking a better solution for sample observations. The Neoc-Neo Osmium Coater- is an innovative instrument which fully covers a sample with highly pure osmium in its large negative glow discharge region. With our own discharge conditions, you will be able to see The complicated surface is fully covered the true structures of your samples. with osmium. No charge-up is seen. The photos provided by Dr. Masaki Ueno (School of Allied Health Sciences, Kitazato University)

Customers’ Comments Customers’ Comments Application Case 1: AFM Cantilever Application Case 3: Nanofibers (Assembled Pigment Molecules) “Osmium Coating Strengthens Cantilevers!” “After Coated by Neoc, Nanofibers Reveal More Details on Nano Scale.” The tips of SPM/AFM cantilevers are applied with a The SEM images below are nanofibers that resulted from various self-assembled pigment molecules such as very strong electric field according to calculations. merocyanine, perylene bisimide and etc. These nanofibers are spontaneously formed at normal temperature under Therefore, Au coated cantilevers easily become normal pressure in a certain period of time after accurately designed pigment molecules are dissolved in a solvent. useless since the tips are severely damaged after a couple of measurements. These pigments self-assemble in three phases (molecule, nano, and micro levels). By traditional coating methods, However, cantilevers coated by the Neoc are much observations at high magnification are difficult due to granularity and poor quality of conductive films. Furthermore, mo re r e s i s t an t t o d amage c au sed du r i ng nanofibers made of pigment molecules are susceptible to heat, and easily deformed by electron beams. Charge-up measurement. These cantilevers can be reused after has been an issue. c l ean ing and recoa t i ng w i th osm ium, and repeatability is still high. It saves a lot of cost. I would like to recommend this coating procedure to The Neoc enables me to observe nanofibers in fine detail at high magnification. Seemingly one fiber turned out to be AFM users. a collection of fine fibers (100 nm). Nanofibers are expected to be utilized as optical/electronic materials made from organic materials in the future. Dr. Toru Nakamura (Advanced Industrial Science and Technology) Dr. Fumiki Yagai (Chiba University) SEM Image of AFM Cantilever Tip Enlarged Application Case 2: TEM Grid Support Film “Pure Osmium Coating is the Best Solution for Sample Support Films.” x 2,000 x 10,000 100nm Nanofibers of Assembled Perylene Bisimid Support films are used when fine particles such as powders and dusts are observed by TEM. Typically, support films are made of collodion or carbon. However, pure osmium has caught a lot of attention recently as it can form a thin but strong film. The properties below make osmium suitable for TEM observation: 1) Strong against electron beams 2) Able to form support films without winkles on a surface x 7,000 x 33,000 x 100,000 3) Able to evenly cover a sample TEM Grid Support Film Nanofibers of Assembled Merocyanine It solves sample drifting issues as well. x 8,500 x 43,000 x 110,000 Cross-section Image of Nanofibers

EM Images of Various Samples Coated EM Images of Various Samples Coated by Neoc by Neoc Sepharose Membrane Filters Sepharose is a type of polymer beads and used for adsorption of biological samples such as proteins and microorganisms. Membrane filters are easily damaged by heat. But the Neoc causes no heat damage to them. The images below show porous structures on the surface. x 2,500 x 50,000 x 10,000 x 50,000 Coal Ash Wolf Skin Hair The sample below is coal ash in cement materials. It has an extremely uneven surface. However, with osmium coating, The images below show wolf skin hair. No heat damage is seen on the sample. These frayed fine hairs are susceptible to you can surely see the details. No charge-up is seen even at 150K magnification. heat. Sputter coating causes this type of samples to get frizzled. x 10,000 x 150,000 x 1,000 x 5,000

EM Images of Various Samples Coated by Neoc Difference Between Gold Sputter and Neoc Drosophila Replicas of Rat Cardiac Blood Vessels No damage is seen in the images below even at 100K magnification. Such complicated samples can be observed without granularity and heat damage. Au Sputter Neoc Head x1,000 Au Coating 3 min Os Coating 20 sec Accelerating Volatge 5kV Accelerating Volatge 5kV x 300 x 300 In order to prevent charge-up, sputter coating needs three minutes to deposit Au on a complicated sample such as replicas of rat cardiac blood vessels. Therefore, samples get shrunk and damaged by heat. Its thick coating makes samples swollen. In contrast, coating by the Neoc only takes 20 seconds. It can form a thin film on a sample without Antenna x 10,000 Antenna x 100,000 charge-up and reveal the original structure in detail. Fossils Au Sputter Neoc Highly porous samples such as fossils can be clearly observed in detail. Au Coating 3 min Os Coating 20 sec Accelerating Volatge 5kV Accelerating Volatge 5kV x 6,000 x 6,000 These are 6K magnified images of replicas of rat cardiac blood vessels. The surface of the Au coated sample is damaged by heat. The difference between sputter and Neoc coating is obvious. x 100,000 x 50,000 Images Provided by Dental School of Medicine, Okayama University