Automated Cell Culture System

2023 Automated Cell Culture System Catalog Creating the Future of Medical Care Equipment with diverse applications: Automated medium change Automated cell passage Cell observation What is Cell Cultivation? ① Changing culture medium daily Process Flow of Automated Cell Culture System Suctioning and removing old culture medium, without peeling off cells, Medium and adding new culture medium change （Cell nutrition） • Maintaining cells’ ② Conducting cell passages every few days Cell environmental temperature Uniformly disseminating proliferated cells into multiple culture vessels according • Controlling culture medium temperature proliferation • Recording of cell images to desired density • Maintaining temperature of culture medium used Requires much time for training Requires continuous work • Removing old culture medium • Cleaning cells by adding buffer solution • Supplying new culture medium Cell passage • Removing cells with exfoliating agent Significant burden on researchers • Collecting cells by centrifuge separation （Cell division） • Dividing cells to new culture vessels Automated cell culture system that continuously supplies cells ▲ Medium dispensing ＆ disseminating Removing supernatant Centrifuging Cell peeling ＆ collecting

Basic Functions for Automating Cell Culturing Processes Clean cabinet Refrigerator ＆ Warmer HEPA filter keeps clean and safe Refrigerators can store environment equivalent to class II Max. 4 500-mL bottles at 4°C. safety cabinet Warmers are provided to warm liquid temporarily. Incubator Cell observation Max. 126 of 100 mm dishes Capable of image saving and simple (or max. 120 of 6-well plates) cell counting can be stored in CO2 incubator Dispensing & Swinging Centrifuging Special 10 mL pipette is A centrifuge is implemented for suitable for separating cells. automated cell passage. Swing equipment to blend Considering the effect of its vibrations suspension in a dish. on cells, it’s placed far from the incubator. Advantages of New System Automating manual techniques All-in-one system Scheduling Compactly consolidating the necessary Reproducing techniques by thoroughly equipment for cell culture, media exchange, and Automatically conducting preset analyzing manual work and utilizing cell passage, including an incubator, observation movements and maintaining history proposed manipulation control technique function, dispensing function, centrifuge, and records of each culture vessel refrigerated storage, into an all-in-one system. Size Cultivating Culture medium change, cell passage, Biohazard Cabinet class II equivalent (Clean 100 equivalent within the unit) process cell observation Partly evaluated according to our own standards Target cell Adhesive cell: ES/iPS cell (colony/single cell), etc. Decontamination UV irradiation, peracetic acid fumigation Heat Cell insulation 4-500 ml bottles observation Image saving, Simple cell counting Culture 100 mm dishes (Max. 126 pcs can be stored) or Main unit W3,000 × D1,390 × H2,412 mm vessel 6-well plates (max. 120 pcs can be stored) dimensions （in case of the large-size incubator） Dedicated ・Three-phase 200 VAC, 30 A: One system supplies 10 mL Tip (Max. 100 bottles) ・100 VAC, 20 A: One system Utilities ・100 VAC, 15 A: Two systems (two 2-pin sockets with a ground) total weight＝about 1,575kg、footprint＝ Cultivation Cultivating process can be set using 1.21×2.78＝3.36m2、 load on the floor＝about 470kg/m2 plan ・CO2 gas supply : One system scheduling function (outer diameter φ6-mm hose barb fitting) Safety Cautions ●Please read the User's Manual carefully to familiarize yourself with safe and effective usage procedures. ●To ensure safety when using this equipment, all work should be performed according to that as stated in the supplied Operating Instructions. Read your operating instruction manual thoroughly. Panasonic Group products are built with the environment in mind. For more information,please visit our website https://www.panasonic.com/jp/company/ppe/saibobaiyo.html Inquiries… Panasonic Production Engineering Co., Ltd. Zip code 571-8502 2-7 Matsubacho, Kadoma,Osaka,Japan; TEL :（+81）6-6905-4882 mail : business.dev@gg.jp.panasonic.com The contents of this catalog are as of September 20, 2023. Ver.September 1, 2023 © Panasonic Production Engineering Co.,Ltd. 2023 ●The color of the product is printed matter and may differ slightly from the actual color. ●The rating and design of the product are subject to change without notice for the purpose of improvement. ●This product is designed for use in Japan.Consult your dealer for overseas use. ●Be sure to read the instruction manual carefully before using the product. ▲ ▲ ▲