Automated Cell Culture and Differentiation System

2025 Automated Cell Culture and Differentiation System Catalog Shaping the Future of Medicine with the Power of Manufacturing All-in-One Automated Cell Culture System Streamline Your Workflow with a Unified Platform Ideal for a Wide Range of Applications ・ Fully Integrated Automation: Automates cell culture, ・ Development of Differentiation Protocols and Reagents: high-precision pipetting, and image analysis in a single system. Accelerate R&D with reproducible and scalable workflows. ・ Smart Data Management: Centralized control and tracking of ・ Preclinical Data Collection & Validation: Generate reliable data for experimental data for enhanced reproducibility. early-stage research and testing. ・ Consistent Quality & Efficiency: Reduces variability and ・ Production of Specific Cell Types: Efficiently culture and supply target manual errors, ensuring high-quality results every time cells for downstream applications.

Application Medium change Preparing the new Removing the old Supplying a new culture medium culture medium culture medium Warm up the new medium to the Remove the old mediumwith the Supply the prepared medium right temperature pipette attached to the arm The dish is properly tilted for efficient addition/removal of the medium while keeping the stress to the cells minimal Cell passage Collecting cells Removing supernatant Counting cells Seeding cells Remove the old medium and Precipitate cells with a centrifuge Count the cells and dilute the Seed cells in new culture dishes clean cells with PBS. Then, and remove supernatant solution to the correct concentration and start the new culture add an enzyme to detach cells Differentiation iPS cell Differentiation Cell Maintenance of differentiation preparation ection xpan- Step1 Step2 Step3 Cell s E Step1 sion passage Step4 el culture Step2 Day-1 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 *Sample timeline based on current use cases, including the establishment of cardiomyocytes, neural progenitor cells, hepatocytes, and keratinocytes. *Actual steps and timelines may vary depending on the cell type and methods ＜Automated and efficient sample preparation＞ Precision liquid handling with accuracy down to a few microliters not only streamlines the process but also significantly reduces reagent waste - an essential benefit when working with growth factors Medium preparation Growth factor Pipetting Supply preparation Growth factor GF dilution

Features Small footprint for better handling： ・The compact design allows for easy loading onto most freight elevators Data management and traceability： • Automated tracking system to streamline the data management and analysis across multiple series of experiments • Ensuring data traceability to help customers comply with GLP and GMP • The user authentication function provides a secure environment and another layer of traceability User-friendly software and interface： • Easy-to-use software with pre-installed methods to meet users’ needs • Visual programming tools to implement protocols without any coding Designed for safety： • A multi-modal safety system integrates computer vision and other sensor data to proactively prevent incidents ‒ no matter the cause, whether due to human error, technical problem, or environmental factors • A remote monitoring system alerts users when intervention or attention is required Remote monitoring system Three monitoring cameras detect and track all dishes, The laser sensor prevents collisions well plates, tubes, and pipette tips throughout each inside the system Cell culture device management system process Automated Master cell culture maintenance Email addresses and notification system website conditions are registered Users are notified Error when an error occurs notification mail Email Linked to DB notification data DB application Regular Checking data every minute notification Users are notified mail at the designated time Sending emails Receiving emails Mail server Email recipient Functionally implementing the “Skilled Researcher”： • Four types of arms, each exceeding ISO 8655 precision standards, deliver exceptional accuracy • The enhanced calibration of pipette tips enables the precise detection of liquid surfaces • A proprietary pipetting workflow, inspired by the sophisticated techniques of experienced researchers, ensures consistent and reproducible results 10 ml 1 ml 200 µl 20 µl Proprietary pipetting workflow Phase-contrast microscope Cell counting Imaging： • Cell counting from phase contrast images • Easy to follow calculation for the specified density * Other features will be added soon

Product Specifications Category Item Specifications Dimensions 3,420 mm (W) × 1,000 mm (D) × 1,950 mm (H) Weight Approx. 2,200 kg Overview Biosafety measures Equivalent to Class II biosafety cabinets (ISO 5 cleanroom) Noise Level Less than 70dB *(typically 60-65dB) Other On-board computer UPS to address sudden power failures Dedicated racks for 100mm dishes and well plates. ①Incubator (Up to four racks, each rack stores x18 100 mm dishes or x18 well plates) Standard settings : Internal temperature: 37 ℃, CO2 level : 5 % ②Imaging Phase-contrast microscope (LED lighting), with auto focus feature ③Tilting X-Y (2D) tilting ④Centrifuge 1 - 1,500 rpm (approx. 400 G), 15 mL or 50 mL tube ⑤Plate / dish supply Dedicated racks for each cell culture plate/dish. (Up to two racks, each rack stores x7 100 mm dishes or x 6 well plates/rack) ⑥Heating 37 ℃. (x4 50 mL and x1 15 mL tubes) 4±2 ℃ Main unit ⑦Refrigerator Four dedicated racks. The default package includes: x1 500 mL bottle rack that stores x1 bottle; x1 1.5 mL tube rack of the system that stores 16 tubes; x2 50 mL tube rack that stores 4 tubes.Option racks are available for 15mL tubes (stores 9 tubes per unit). ⑧Tube storage Automatic feed : x1 50 mL tube, x1 15 mL tube, and x6 1.5 mL tubes Manual feed : x2 50 mL tubes, x2 15 mL tubes, and x3 1.5 mL tubes Dedicated racks for Panasonic pipette tips. (Up to four racks, each rack stores x4 10 mL tips (100 tips in total)) ⑨Pipette tip supply 20 µL, 200 µL, and 1-mL pipette tips are also available (96 pcs/case, each rack stores x2 each type of tips). ⑩Tube supply x24 50 mL, x18 15 mL, and x32 1.5 mL tubes. Liquid Handling X-Y-Z (3D) and Y-Z (2D) arms, equipped with interchangeable units (for dishes and well plates, tubes, 20 µL/200 µL/1mL pipette tips, and 10 mL pipettes) Operation unit Dedicated control panel and PC for operation Other UV lamps for disinfection, Cell counting jig, and autoclavable waste tank (5L). Power supply 3-phase AC 200 V, 60 A, single line CO2 supply 0.1 ± 0.05 MPa, outer diameter 6 mm air tube Installation environment Air conditioning Temperature : 23 ± 3 ℃, Relative Humidity: 35 ‒ 70 %, Free from dust, oil mist, and corrosive gases Installation floor Floor levelness : ±25 mm, ±2 mm/m ; Floor load capacity : 640 kg/m² Other Not required (Main unit equipped with 6 sets of casters and adjustable feet), No floor vibration or electrical noise sources 4 1 2 3 6 8 7 9 10 5 Safety Cautions ●Please read the User's Manual carefully to familiarize yourself with safe and effective usage procedures. ●To ensure safety when using this equipment, all work should be performed according to that as stated in the supplied Operating Instructions. Read your operating instruction manual thoroughly. Panasonic Group products are built with the environment in mind. For more information,please visit our website ▲ https://www.panasonic.com/jp/company/ppe/saibobaiyo.html Inquiries… Panasonic Production Engineering Co., Ltd. Zip code 571-8502 2-7 Matsubacho, Kadoma,Osaka,Japan; TEL （: +81）6-6905-4882 The contents of this catalog are as of September 1, 2025. Ver.September 1, 2025 © Panasonic Production Engineering Co.,Ltd. 2025 ●The color of the product is printed matter and may differ slightly from the actual color. ●The rating and design of the product are subject to change without notice for the purpose of improvement. ●This product is designed for use in Japan.Consult your dealer for overseas use. ●Be sure to read the instruction manual carefully before using the product.